Journal: bioRxiv
Article Title: Investigating the mechanisms of indocyanine green (ICG) cellular uptake in sarcoma
doi: 10.1101/2021.04.05.438013
Figure Lengend Snippet: Scatter plot showing the correlation between flow cytometry median fluorescence intensity of ICG; 635 780/60 (y-axis) and cell line proliferation rate; CCK-8 (x-axis). Labelled data points indicate each cancer cell line, Mean ± SEM, with linear trend line (dotted blue). Correlation was calculated using SPSS software (V.27) non-parametric Spearman’s rho function; Rs = 1.000, p<0.001 (2-tailed). The analysed MFI and CCK data were taken as the mean value over three experiments, repeated on separate occasions. MFI= median fluorescence intensity, CCK-8= cell counting kit-8, SEM= standard error of the mean , ICG= indocyanine green.
Article Snippet: Cellular proliferation rate was assessed using Cell Counting Kit-8 (CCK-8, Dojindo Labatories, CK04-11) as per the manufacturer’s manual.
Techniques: Flow Cytometry, Fluorescence, CCK-8 Assay, Software, Cell Counting